Journal of Cancer Biology and Clinical Oncology

Abstract

HAI-1 Over-Expression Promotes Autophagy of Prostate Cancer PC-3 Cells

Author(s): Chuan-Yi Hu, Juan Chen, Jing-Cun Zheng, Jing Zhang, Guo-Zeng Wang, Ning Jiang

This study examined the potential effect of HAI-1 over-expression on autophagy of prostate cancer PC-3 cells and its possible mechanism. PC-3 cells were transfected with plasmids to generate HAI-1 stably over-expressing PC-3/HAI-1 and control cell lines. The relative levels of HAI-1 mRNA transcripts in control PC-3 and PC-3/HAI-1 cells were determined by RT_PCR. The levels of Beclin1 expression in both cell lines were determined by Western blot. The levels of autophagic vesicles and autophagosomes in these cell lines were characterized by Cyto- ID® Autophagy Detection Dye Staining and transmission electronic microscopy. In comparison with the control PC-3 cells, HAI-1 mRNA transcripts and Beclin1 expression significantly increased in PC_3/HAI1 cells. There were many autophagic vesicles and autophagosomes detected in PC-3/HAI-1 cells. In conclusion, HAI-1 over-expression may promote autophagy of prostate cancer PC-3 cells in vitro.

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