Author(s): Cuero RaulThe aim of this investigation was to recognize SARS-CoV-2 antibodies In vitro using synthetic biology. A DNA sensor was constructed using natural and synthetic genetic sequences in SHuffle T7 Express competent bacterial or yeast cells. These sensors were based on expression of SARS-CoV-2 proteins that includes the spike protein, the membrane glycoprotein, the nucleocapsid protein, and 3CL protease. Angiotensin-converting enzyme 2 (ACE 2) and enhanced green fluorescent protein (EGFP) were also added to strengthen detection capability of these sensors. Genes for these proteins were assembled and hosted in bacteria or yeast. Lysates from transformed organisms were used as the source of antigens for recognition by SARS-CoV-2-specific commercial antibodies using ELISA method. Construction of the COVID-19 antibody sensor was also confirmed by using Raman spectrophotometric analysis to corroborate the expression of the recombinant proteins related to spike and nucleocapsid. These results were compatible with the SARS-CoV-2 proteins developed into the DNA sensor.