Integrative Molecular Biology and Biotechnology


Optimization and Initiation of Merstematic Tissue in Melicope Lunu-Ankenda for Organ Culture Establishment

Author(s): Zuraida Ab Rahman, Ayu Nazreena Othman, Chandradevan A/L Machap, Nur Najwa Arifah Basiron, Amirah Balqis Amir Amran and Nurul Qistina Mohamed Bakri

The meristematic tissue or callus induction was observed in culture medium consisted MS basal medium, 3% (w/v) sucrose, 3 g/L phytogel, supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) (0, 0.5, 1.0, 3.0 or 5.0 mg/L) and α-Naphthalene acetic acid (NAA) (0, 1.0, 3.0, 5.0 or 10 mg/L) either on its own or combined. At 1.0 mg/L 2,4-D with combination of 10.0 mg/L NAA, dark green meristematic tissue was produced with the greatest weight, 8.45 g. This treatment showed the best characteristics which is dark green colour, highest in weight of and the low number of shoots produced. Meristematic tissue that cultured in a liquid shake media also established. The combination of BAP (0.5 – 1.0 mg/L) and NAA (0.5 – 1.0 mg/L) was superior to other treatments for producing fresh weight of cultures with a maximum of 12.67 g. More meristematic tissue with unorganized cells was produced rather those plantlets in this treatment which can be utilized further in organ culture establishment.

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