Journal of Neurology and Neuro Toxicology

Abstract

Neuroprotective Effects of Bone Marrow Mesenchymal Stem Cells Combined with Ginsenoide Rb1 on Mice with Intracerebral Hemorrhage

Author(s): Wei Wang, Ling Yin, Kan Guo, Li Deng, Xiaoqing Gao, Jiaxing Pan, Yan Zhu, Chaoxian Yang

Aim of the study: To investigate the neuroprotective effects of bone marrow mesenchymal stem cells (BMSCs) combined with ginsenoide Rb1 (GRb1) on mice with intracerebral hemorrhage (ICH). 
Methods: C57BL/6 mice with ICH were divided into control group, GRb1 group, BMSCs group and BMSCs +GRb1 group randomly, then each group was subdivided into 14d and 28d subgroups. BMSCs and GRb1were injected 2 days after ICH. Modified neurological severity scores (mNSS) were used to evaluate the neurological function. Hoechest 33258 staining was used to detect the apoptotic cells and hematoxylin and eosin (H&E) staining was applied to observe the morphological changes of brain tissues. Immunofluorescence was used to assess the differentiation of transplanted BMSCs in vivo. 
Results: In four groups, BMSCs+Rb1 group has the lowest mNSS scores and the minimum number of apoptotic cells (p<0.05). Compared with the control group, the degrees of lesion were reduced and the morphological structures were more complete in other three groups. Compared with the BMSCs group, the positive percentages of GFAP (on day 28), MAP2 and nestin ( on day 14 and 28) among grafted cells were significantly increased in BMSCs+GRb1 group (p<0.05). 
Conclusion: Combined treatment of BMSCs transplantation and GRb1 provide better neuroprotection for mice with ICH.

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