Veterinary Sciences and Medicine

Abstract

Molecular Detection and Evaluation of Some Immunological Parameter on Salmonellosis Patients in Al-Muthanna Province

Author(s): Tareq Jaffaar Al-jindeel and Ihsan Abdullah Kumail

One hundred samples were collected from human blood depended on age, gender, region, taking medication, and smoking during the extended period from December/ 2015 to July/ 2016 in Al Hussein Teaching Hospital of Al-Muthanna Province. Forty-five samples were positive to Widal test as titer ≥ 1:320. Real Time Polymerase Chain Reaction technique was done on DNA samples extracted from 45 positive Widal test fresh blood samples, that’s done by using Flic as diagnostic gene and SYBR Green I dye. The results showed 21 (46.6 %) samples were positive and 24 (53.3 %) were negative. The nitro blue tetrazolium (NBT) test was done to estimate the phagocytic activity in positive Widal test patients. The results of (NBT) test showed a significant increase (P<0.05) in phagocytic activity in the blood of patients who had positive Widal test, compared with the blood of healthy individuals (79.3%) as a positive control. The MTT assay was used for evaluating the lymphocyte transformation index % of peripheral blood leukocytes in positive Widal test patients. The results of MTT assay showed a significant increase (P<0.05) in the lymphocyte transformation index in the lymphocytes of positive Widal test patients, compared with the lymphocytes of healthy individuals (75.6%) as a positive control.

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