Author(s): Navarro C, Arros M, Jara MA, Mosnaim ADNosocomial bacteria acquisition of resistance to multiple antimicrobial agents is a critical concern in medical practice since may result in patient’s increased morbidity and mortality. Additionally, it causes an increased cost to the patient and medical centers as it generates complications and a longer hospital permanency. The information that allows bacteria to develop mechanisms of resistance is found in its genome, having the ability to be transferred in horizontal way to other bacteria. In order to improve pathogen control protocols associated with this type of infection we need to develop a procedure for the dynamic tracing of nosocomial bacterial species, establishing a constant feedback of information regarding their existence, and the characteristics of the environmental responsible for their appearance. This protocol should include identifying the genes responsible for antimicrobial resistance in different species and an analysis of their epidemiological relationship, looking for safeguarding public and animal health.