Author(s): Sadam, D. Hasan and Kamal. M. AlsaadThis is a pioneering molecular report investigating the genetic diversity and phylogenic analysis of BVDV in cattle of Nineveh province, Iraq. A total of 494 ear notch samples was collected from cattle in five different regions of Nineveh province and tested by multiplex PCR assay. The overall prevalence of BVDV genotypes was 13.96% (69 out of 494), for BVDV1 it was 12.95% and for BVDV2 it was 1.01%. Bovine viral diarrhea virus 1 was significantly more detected than BVDV2 (P< 0.05). The individual sequencing analysis (BLASTn) of 69 sequences of 5’UTR gene, revealed for the first time the existence of three sub genotypes of BVDV1 in Nineveh province: sub genotype A, B and E were 3.44%, 7.89%, and 1.61% respectively. BVDV 1 sub genotype B was the most commonly detected in cattle (P<0.05). In addition, for the first time, two sub genotypes of BVDV2 were found in Nineveh province (sub genotype A 0.60% and sub genotype B 0.40%). Bovine viral diarrhea virus 2 sub genotype A was commonly detected in cattle. It has been concluded that the current study found the genetic diversity of BVDV which circulating among the cattle population in Nineveh province and this result could have a significant implications for the epidemiology, diagnosis and control of the disease in Iraq.