Author(s): Victoria C Lorca, Consuelo F Borie, Carlos O NavarroThe canine Brucellosis (BC) is an infectious, contagious and zoonotic disease caused by Brucella canis (B. canis). Nevertheless, there have been described sporadic infections caused also by Brucella sues and Brucella aborts as also for Brucella melitensis which produce an auto limited illness. This infection is characterized for producing infertility in males and females, affecting the reproductive life of the animal, which leads to important economic losses in breeding-kennels and affective loss for owners when the dog it carried to euthanasia. The diagnosis is usually realized by means serological tests whose principal disadvantage is the production of false positive results due to crossed reactivity with other bacteria, of the same or different genus, or false negative results in chronic infection cases and for this reason the diagnostic confirmation by means bacterial isolation is necessary. Nevertheless, the previous carries out risk of infection to the laboratory personnel due to zoonotic character of these bacteria. Additionally, this procedure involves a long period of incubation.